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Can I read Simultaneous Liquid Chromatographic Determination of Ribonucleoside-5′-monophosphates and Their Isomers in Potato Tubers on EtoBox?
Simultaneous Liquid Chromatographic Determination of Ribonucleoside-5′-monophosphates and Their Isomers in Potato Tubers by T.T. Nguyen; P. Sporns; D. Hadziyev is a Chemistry article available to read on EtoBox.
What is Simultaneous Liquid Chromatographic Determination of Ribonucleoside-5′-monophosphates and Their Isomers in Potato Tubers about?
A liquid chromatographic method has been developed for simultaneous determination of flavor-enhancing nucleotides (guanosine-5'-monophosphate and inosine-5'-monophosphate) along with other RNA breakdown products from raw and processed potatoes. The method consists of potato tuber homogenization in extracting solution of 0.5 M perchloric acid, removal of starch by addition of methanol and centrifugation. Prior to injection to a liquid chromatography column (Whatman Partisil SAX), the perchloric acid is removed from the extract by treatment with tri-noctylamine-Freon 113 solution. With isocratic conditions using a solvent of 3% methanol and 0.008 M phosphate buffer (pH 4.15) the nucleotides present in potato tubers could be accurately quantitated.
Who reads Simultaneous Liquid Chromatographic Determination of Ribonucleoside-5′-monophosphates and Their Isomers in Potato Tubers?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- T.T. Nguyen; P. Sporns; D. Hadziyev
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 1986
- Language
- EN
- Field
- Chemistry (Physical Sciences)