Can I read Cytochrome P450 Protocols (Methods in Molecular Biology (320)) on EtoBox?

Cytochrome P450 Protocols (Methods in Molecular Biology (320)) by Phillips, Ian R. (Ian Randall), 1951-; Shephard, Elizabeth A. (Elizabeth Anne), 1950- is a nonfiction available to read on EtoBox.

What is Cytochrome P450 Protocols (Methods in Molecular Biology (320)) about?

For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Also described are techniques for the generation of mice with targeted gene disruptions. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source. Each chapter is written by researchers who have been involved in the development and application of the particular technique to P450s or FMOs. The protocols are presented in a step-by-step manner, with extensive cross-references to notes that highlight critical steps, potential problems, and alt

Who reads Cytochrome P450 Protocols (Methods in Molecular Biology (320))?

Author

Phillips, Ian R. (Ian Randall), 1951-; Shephard, Elizabeth A. (Elizabeth Anne), 1950-

Publisher

Humana Press : Imprint : Humana Press

Published

2006

Language

EN

ISBN

9781592599981

Category

nonfiction

Subjects

Science, Chemistry, Medical