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A Simple Method for the Production of Low Molecular Weight Hyaluronan by in Situ Degradation in Fermentation Broth by Jianfeng Mei; Zhihong Dong; Yu Yi; Yanlu Zhang; Guoqing Ying is a Materials Science article available to read on EtoBox.

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## Abstract Fermentation of hyaluronan (HA) by __Streptococcus zooepidemicus__ was carried out in a 10-L fermentor. When the medium pH was controlled at 7.0 and the temperature was maintained at 38°C for 12 h followed by 35°C for 8 h, the yield of HA was 4.83 g/L with a molecular weight of 1,890 kDa. After the cells were removed by centrifugation from the fermentation broth, HA was slowly degraded to low molecular weight HA by hyaluronidase at a suitable temperature without a decrease in HA concentration. If the time and temperature for enzymatic degradation were controlled, the desired low molecular weight HA could be obtained by __in situ__ degradation in the fermentation broth. The method does not require the addition of exogenous hyaluronidase, and is a simple way to produce low molecular weight HA.

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Author

Jianfeng Mei; Zhihong Dong; Yu Yi; Yanlu Zhang; Guoqing Ying

Publisher

Walter de Gruyter GmbH

Published

2019

Language

EN

Field

Materials Science (Physical Sciences)