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Comparative study on the effect of different drying techniques on phenolic compounds in Australian beach-cast brown seaweeds 1 1 Introduction 1 2 Materials and methods 2 2.1 Chemicals 2 2.2 Seaweed collection and identification of seaweed samples 2 2.3 Sample preparation 2 2.3.1 Preparation of dried samples 2 2.3.2 Extraction preparation 3 2.4 Estimation of phenolic compounds and antioxidant assay 3 2.4.1 Determination of total phenolic content (TPC) 3 2.4.2 Determination of total flavonoid compounds (TFC) 3 2.4.3 2,4-Dimethoxybenzaldehyde assay (DMBA) 3 2.4.4 Prussian blue assay (PBA) 3 2.4.5 Folin-Denis Assay (FDA) 3 2.4.6 2,2′-Diphenyl-1-picrylhydrazyl (DPPH) Assay 3 2.4.7 Ferric Reducing Antioxidant Power (FRAP) assay 3 2.4.8 2,2′-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay 4 2.4.9 Estimation of hydroxyl radical scavenging activity (·OH-RSA) 4 2.4.10 Estimation of ferrous ion chelating activity (FICA) 4 2.4.11 Estimation of reducing power (RPA) 4 2.4.12 Total antioxidant capacity (TAC) 4 2.5 Characterization of phenolic compounds by LC-ESI-QTOF-MS/MS analysis 4 2.6 Statistical analysis 4 3 Results and discussion 4 3.1 Estimation of phenolic content 4 3.2 Antio

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EN

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nonfiction