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Can I read DNA methylation‐mediated silencing of nonsteroidal anti‐inflammatory drug‐activated gene (NAG‐1/GDF15) in glioma cell lines on EtoBox?
DNA methylation‐mediated silencing of nonsteroidal anti‐inflammatory drug‐activated gene (NAG‐1/GDF15) in glioma cell lines by Mitsutoshi Kadowaki; Hiroki Yoshioka; Hideki Kamitani; Takashi Watanabe; Paul A. Wade; Thomas E. Eling is a Medicine article available to read on EtoBox.
What is DNA methylation‐mediated silencing of nonsteroidal anti‐inflammatory drug‐activated gene (NAG‐1/GDF15) in glioma cell lines about?
## Abstract Nonsteroidal anti‐inflammatory drug‐activated gene, __NAG‐1__, a transforming growth factor‐β member, is involved in tumor progression and development. The association between NAG‐1 expression and development and progression of glioma has not been well defined. Glioblastoma cell lines have lower basal expression of NAG‐1 than other gliomas and normal astrocytes. Most primary human gliomas have very low levels of NAG‐1 expression. NAG‐1 basal expression appeared to inversely correlate with tumor grade in glioma. Aberrant promoter hypermethylation is a common mechanism for silencing of tumor suppressor genes in cancer cells. In glioblastoma cell lines, NAG‐1 expression was increased by the demethylating agent, 5‐aza‐2′‐deoxycytidine. To investigate whether the __NAG‐1__ gene was silenced by hypermethylation in glioblastoma, we examined DNA methylation status using genomic bisulfite sequencing. The NAG‐1 promoter was densely methylated in several glioblastoma cell lines as well as in primary oligodendroglioma tumor samples, which have low basal expression of NAG‐1. DNA methylation at two specific sites (−53 and +55 CpG sites) in the NAG‐1 promoter was strongly associated w
Who reads DNA methylation‐mediated silencing of nonsteroidal anti‐inflammatory drug‐activated gene (NAG‐1/GDF15) in glioma cell lines?
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- Author
- Mitsutoshi Kadowaki; Hiroki Yoshioka; Hideki Kamitani; Takashi Watanabe; Paul A. Wade; Thomas E. Eling
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 2011
- Language
- EN
- Field
- Medicine (Health Sciences)