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Protective Effects of Human Liver Stem Cell-Derived Extracellular Vesicles in a Mouse Model of Hepatic Ischemia-Reperfusion Injury by Alberto Calleri; Dorotea Roggio; Victor Navarro-Tableros; Nicola De Stefano; Chiara Pasquino; Ezio David; Giada Frigatti; Federica Rigo; Federica Antico; Paola Caropreso; Damiano Patrono; Stefania Bruno; Renato Romagnoli is a Medicine article available to read on EtoBox.

What is Protective Effects of Human Liver Stem Cell-Derived Extracellular Vesicles in a Mouse Model of Hepatic Ischemia-Reperfusion Injury about?

Hepatic ischemia-reperfusion injury (IRI) is observed in liver transplantation and hepato-biliary surgery and is associated with an inflammatory response. Human liver stem cell-derived extracellular vesicles (HLSC-EV) have been demonstrated to reduce liver damage in different experimental settings by accelerating regeneration and by modulating inflammation. The aim of the present study was to investigate whether HLSC-EV may protect liver from IRI in a mouse experimental model. Segmental IRI was obtained by selective clamping of intrahepatic pedicles for 90 min followed by 6 h of reperfusion. HLSC-EV were administered intravenously at the end of the ischemic period and histopathological and biochemical alterations were evaluated in comparison with controls injected with vehicle alone. Intra liver localization of labeled HLSC-EV was assessed by in in vivo Imaging System (IVIS) and the internalization into hepatocytes was confirmed by fluorescence analyses. As compared to the control group, administration of 3 × 10^9^ particles (EV1 group) significantly reduced alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) release, necrosis extension and cytokines expression (TNF-α, C

Who reads Protective Effects of Human Liver Stem Cell-Derived Extracellular Vesicles in a Mouse Model of Hepatic Ischemia-Reperfusion Injury?

Author

Alberto Calleri; Dorotea Roggio; Victor Navarro-Tableros; Nicola De Stefano; Chiara Pasquino; Ezio David; Giada Frigatti; Federica Rigo; Federica Antico; Paola Caropreso; Damiano Patrono; Stefania Bruno; Renato Romagnoli

Publisher

Humana Press Inc; Springer-Verlag; Springer Verlag; Humana Press, Inc.; Springer Science and Business Media LLC (ISSN 1550-8943)

Published

2020

Language

EN

Field

Medicine (Health Sciences)