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Rapid Identification of Siderophores by Combined Thin-layer Chromatography/matrix-assisted Laser Desorption/ionization Mass Spectrometry by Heiko Hayen; Dietrich A. Volmer is a Chemistry article available to read on EtoBox.

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## Abstract The investigation of a combined thin‐layer chromatography/matrix‐assisted laser desorption/ionization mass spectrometry (TLC/MALDI‐MS) method for the analysis of siderophores from microbial samples is described. The investigated siderophores were enterobactin, ferrioxamine B, ferrichrome, ferrirhodin, rhodotorulic acid and coprogen. Solid‐phase extraction was employed to recover the siderophores from the microbial samples. After visualization of the spots via spraying with ferric chloride or chrome azurol sulfonate assay solution, the MALDI matrix was applied to the gel surface. Several TLC/MALDI experimental parameters were optimized, such as type and concentration of MALDI matrix, as well as the type and composition of solvent to facilitate analyte transport from the inside of the TLC gel to the surface. The impact of these parameters on sensitivity, precision and ion formation of the various siderophores was studied. The detection limits for the investigated siderophores were in the range 1–4 pmol. These values were about 4–24 times higher than the detection limits obtained directly from stainless steel MALDI targets. The differences were most likely due to incomplet

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Author

Heiko Hayen; Dietrich A. Volmer

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2005

Language

EN

Field

Chemistry (Physical Sciences)