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Zero amylose waxy (CDC Alamo), normal (Falcon), and high amylose hull-less barley starches were isolated and subjected to -amylase (Bacillus licheniformis) hydrolysis (24 and 48 h; 50 and 500 U/g) and acid hydrolysis (2% HCl, 10 and 96 h) in diVerent alcohol media (methanol, ethanol, and 1-butanol) to determine the molecular characteristics of the starch material remaining in the granules after treatments. The greatest yield of -amylase-treated starches was obtained for the high amylose (79.9-86.6% w/w) followed by normal (61.1-76.1% w/w), and zero amylose waxy starch (44.2-64.5% w/w). Very little solubilization of starches occurred during acid/alcohol hydrolysis, and very high yields of treated starches were obtained (91-99%). Relatively small decreases in the average molecular weights (M w ) of starch components were observed after -amylolysis; the extensive hydrolysis of the zero amylose starch (56% solubilization) caused only a threefold decrease in the M w of amylopectin. The M w of starch fractions after acid/alcohol hydrolysis was aVected by the hydrolysis time and alcohol but not by the amount of amylose in the native starches; the M w and the mode of distribution of variou

Author

S. You; M.S. Izydorczyk

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0144-8617)

Published

2007

Language

EN