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Separation of plant viral satellite double-stranded RNA using high-performance liquid chromatography by Saša Likić; Gordana Rusak; Mladen Krajačić is a scholarly article available to read on EtoBox.

What is Separation of plant viral satellite double-stranded RNA using high-performance liquid chromatography about?

High-performance liquid chromatography was developed for further separation of double-stranded (ds) RNAs obtained by CF-11 cellulose chromatography from plants infected with satellite associated cucumber mosaic virus. Fractions separated by monolithic polymer column, especially applicable for nucleic acid analyses, were identified electrophoretically and confirmed with a polymerase chain reaction test. Once standardized, the method has revealed clear evidence of satellite presence without precipitation and electrophoresis. According to demonstrated sensitivity, its application in the preliminary diagnostics of field samples is also predictable. Principally, it can be used as a powerful preparative approach resulting in highly pure satellite dsRNA for further analyses.

Author

Saša Likić; Gordana Rusak; Mladen Krajačić

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2008

Language

EN