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Determination of Various Alcohols Based on a New Immobilized Enzyme Fluorescence Capillary Analysis by Yong-Sheng Li; Xiu-Feng Gao is a Chemistry article available to read on EtoBox.
What is Determination of Various Alcohols Based on a New Immobilized Enzyme Fluorescence Capillary Analysis about?
A novel method for the determination of ethanol in tequila based on the immobilized enzyme fluorescence capillary analysis (IE-EFCA) has been proposed. Alcohol dehydrogenase (ADH) was immobilized in inner surface of a capillary and an immobilized enzyme capillary bioreactor (IE-ECBR) was formed. After nicotinamide adenine dinucleotide (NAD + ) as an oxidizer is mixed with alcohol sample solution, it was sucked into the IE-ECBR. The fluorescence intensity of the mixed solution in the IE-ECBR was detected at λ ex = 350 nm and λ em = 459 nm. The experimental conditions are as follows: The reaction time is 20 min; temperature is 40 • C; the concentrations of phosphate buffer solution (pH 7.5) and NAD + are 0.1 mol L -1 and 5 mmol L -1 , respectively; immobilization concentration of ADH is 10 U L -1 . The determination range of ethanol is 2.0-15.0 g L -1 (F = 10.44C + 6.6002, r > 0.9958); its detection limit is 1.11 g L -1 ; and relative standard deviation is 1.9%. IE-EFCA method is applicable for the determination of the samples containing alcohol in medicine, industry and environment.
Who reads Determination of Various Alcohols Based on a New Immobilized Enzyme Fluorescence Capillary Analysis?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Yong-Sheng Li; Xiu-Feng Gao
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)
- Published
- 2007
- Language
- EN
- Field
- Chemistry (Physical Sciences)