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Ribosome Profiling at Isoform Level Reveals Evolutionary Conserved Impacts of Differential Splicing on the Proteome by Reixachs-Solé, Marina; Ruiz-Orera, Jorge; Albà, M. Mar; Eyras, Eduardo is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Ribosome Profiling at Isoform Level Reveals Evolutionary Conserved Impacts of Differential Splicing on the Proteome about?

The differential production of transcript isoforms from gene loci is a key cellular mechanism. Yet, its impact in protein production remains an open question. Here, we describe ORQAS (ORF quantification pipeline for alternative splicing), a pipeline for the translation quantification of individual transcript isoforms using ribosome-protected mRNA fragments (ribosome profiling). We find evidence of translation for 40–50% of the expressed isoforms in human and mouse, with 53% of the expressed genes having more than one translated isoform in human, and 33% in mouse. Differential splicing analysis revealed that about 40% of the splicing changes at RNA level are concordant with changes in translation. Furthermore, orthologous cassette exons between human and mouse preserve the directionality of the change, and are enriched in microexons in a comparison between glia and glioma. ORQAS leverages ribosome profiling to uncover a widespread and evolutionarily conserved impact of differential splicing on translation, particularly of microexon-containing isoforms.

Who reads Ribosome Profiling at Isoform Level Reveals Evolutionary Conserved Impacts of Differential Splicing on the Proteome?

Author

Reixachs-Solé, Marina; Ruiz-Orera, Jorge; Albà, M. Mar; Eyras, Eduardo

Publisher

Nature Publishing Group; Springer Science and Business Media LLC; [London]: Nature Publishing Group, 2010-; Society for Mining, Metallurgy and Exploration Inc.; Research Square (ISSN 2041-1723)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)