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A Fluorescence Aptasensor Based on Two-dimensional Sheet Metal-organic Frameworks for Monitoring Adenosine Triphosphate by Xiao-man Hai; Nan Li; Ke Wang; Zhi-qi Zhang; Jing Zhang; Fu-quan Dang is a Chemistry article available to read on EtoBox.

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In the present study, a facile fluorescence aptasensor based on two-dimensional sheet metal-organic frameworks of N,N-bis(2-hydroxyethyl)dithiooxamidato copper(II) (HdtoaCu) was developed for the sensitive detection of adenosine triphosphate (ATP). The sensing mechanism was based on the noncovalent interaction between FAM-labeled (fluorescein amidite) ATP aptamers and HdtoaCu. In the absence of ATP, the FAM-labeled aptamer readily adsorbs onto HdtoaCu, mainly via π-π stacking and hydrogen bond interactions between the nucleotide bases and the HdtoaCu surface, leading to the reduction of fluorescence intensity of the FAM by photoinduced electron transfer (PET). In the presence of ATP, the FAM-labeled aptamer specifically forms ATP-binding aptamer complexes which exhibit only weak adsorption on the HdtoaCu surface. Thus, the fluorescence of the FAM-labeled ATP aptamer remained largely unchanged. The fluorescence aptasensor exhibited a good linear relationship between the fluorescence intensity and the logarithm concentration of ATP over a range of 25-400 nM, with a detection limit of 8.19 nM (3S/N). ATP analogs such as guanosine triphosphate, uridine triphosphate, and cytidine tripho

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Author

Xiao-man Hai; Nan Li; Ke Wang; Zhi-qi Zhang; Jing Zhang; Fu-quan Dang

Published

2017

Language

EN

Field

Chemistry (Physical Sciences)