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Determination of 8-Methoxypsoralen in Human Plasma, and Microdialysates Using Liquid Chromatography–tandem Mass Spectrometry by Lutz Bräutigam; Maic Seegel; Irmgard Tegeder; Helmut Schmidt; Silke Meier; Maurizio Podda; Roland Kaufmann; Marcella Grundmann-Kollmann; Gerd Geisslinger is a Chemistry article available to read on EtoBox.
What is Determination of 8-Methoxypsoralen in Human Plasma, and Microdialysates Using Liquid Chromatography–tandem Mass Spectrometry about?
The validation of a LC/MS/MS method for the determination of 8-methoxypsoralen (8-MOP) in human plasma and microdialysates after topical application is described. Plasma samples were extracted by liquid-liquid extraction with diisopropylether using 4,5 ,8-trimethylpsoralen (TMP) as internal standard. Chromatographic separation of plasma sample extracts was carried out using a short narrow-bore Nucleosil C18 column (30 mm × 2.0 mm i.d.) with acetonitrile/(2 mM ammonium acetate buffer, 2 mM acetic acid) (80:20, v/v). For mass spectrometric analysis an API 3000 triple quadrupole mass spectrometer was employed. The mass transitions used were m/z 217.2 → 174.0 for 8-MOP and m/z 229.1 → 142.1 for TMP. Microdialysis samples diluted with an equal amount of acetonitrile did not require any extraction and were analyzed directly on a narrow-bore Nucleosil C18 column (70 mm × 2.0 mm i.d.) with acetonitrile/(2 mM ammonium acetate buffer, 2 mM acetic acid) (50:50, v/v) with the mass transition m/z 217.2 → 174.0. The assays were validated over the concentration ranges of 0.5-50 ng/ml for plasma samples and 0.25-50 ng/ml for microdialysates, respectively.
Who reads Determination of 8-Methoxypsoralen in Human Plasma, and Microdialysates Using Liquid Chromatography–tandem Mass Spectrometry?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Lutz Bräutigam; Maic Seegel; Irmgard Tegeder; Helmut Schmidt; Silke Meier; Maurizio Podda; Roland Kaufmann; Marcella Grundmann-Kollmann; Gerd Geisslinger
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-376X)
- Published
- 2003
- Language
- EN
- Field
- Chemistry (Physical Sciences)