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Near Quantitative Ligation Results in Resistance of DNA Origami Against Nuclease and Cell Lysate by Kirankumar Krishnamurthy; Arivazhagan Rajendran; Eiji Nakata; Takashi Morii is a Engineering article available to read on EtoBox.

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## Abstract There have been limited efforts to ligate the staple nicks in DNA origami which is crucial for their stability against thermal and mechanical treatments, and chemical and biological environments. Here, two near quantitative ligation methods are demonstrated for the native backbone linkage at the nicks in origami: i) a cosolvent dimethyl sulfoxide (DMSO)‐assisted enzymatic ligation and ii) enzyme‐free chemical ligation by CNBr. Both methods achieved over 90% ligation in 2D origami, only CNBr‐method resulted in ≈80% ligation in 3D origami, while the enzyme‐alone yielded 31–55% (2D) or 22–36% (3D) ligation. Only CNBr‐method worked efficiently for 3D origami. The CNBr‐mediated reaction is completed within 5 min, while DMSO‐method took overnight. Ligation by these methods improved the structural stability up to 30 °C, stability during the electrophoresis and subsequent extraction, and against nuclease and cell lysate. These methods are straightforward, non‐tedious, and superior in terms of cost, reaction time, and efficiency.

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Author

Kirankumar Krishnamurthy; Arivazhagan Rajendran; Eiji Nakata; Takashi Morii

Publisher

Wiley

Published

2023

Language

EN

Field

Engineering (Physical Sciences)