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Extensive CDR3H Length Heterogeneity Exists in Bovine Foetal VDJ Rearrangements by S. S. Saini; A. Kaushik is a Immunology and Microbiology article available to read on EtoBox.
What is Extensive CDR3H Length Heterogeneity Exists in Bovine Foetal VDJ Rearrangements about?
Analysis of seven variable‐diversity‐joining (VDJ) gene rearrangements in B splenocytes from a 125‐day‐old bovine foetus revealed an extensive heavy‐chain complementarity‐determining region 3 (CDR3H) length variation (9–56 codons). Indeed, the global CDR3H size spectratyping of foetal VDJ rearrangements substantiated such an extensive heterogeneity and was comparable with that noted in peripheral B lymphocytes of adult cattle. These observations are in contrast to species such as humans with extensive germline combinatorial capability where shorter CDR3H length is noted early during B‐cell development. Exceptionally long CDR3H (as in adult cattle) was noted in two foetal VDJ rearrangements encoded by a single germline V~H~ gene. Further, two V~H~ genes (gl.110.20 and BF2B5) were preferentially expressed in the foetal VDJ rearrangements. The D~H~ gene‐encoded CDR3H region of foetal VDJ rearrangements is remarkable for repetitive GGT (glycine) and TAT (tyrosine) codons that favour the recruitment of somatic hypermutations. It appears that closely related germline D~H~ genes, preferentially used in the hydrophilic reading frame, encode varying CDR3H lengths early during B‐cell ontogen
Who reads Extensive CDR3H Length Heterogeneity Exists in Bovine Foetal VDJ Rearrangements?
It is typically read by researchers, students, and practitioners in Immunology and Microbiology.
- Author
- S. S. Saini; A. Kaushik
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0300-9475)
- Published
- 2002
- Language
- EN
- Field
- Immunology and Microbiology (Life Sciences)