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β-PrP form of human prion protein stimulates production of monoclonal antibodies to epitope 91–110 that recognise native PrPSc by Azadeh Khalili-Shirazi; Maria Kaisar; Gary Mallinson; Samantha Jones; Daljit Bhelt; Carol Fraser; Anthony R. Clarke; Simon H. Hawke; Graham S. Jackson; John Collinge is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Prion diseases are associated with accumulation of strain-dependent biochemically distinct, disease-related isoforms (PrP Sc ) of host-encoded prion protein (PrP C ). PrP Sc is characterised by increased β-sheet content, detergent insolubility and protease resistance. Recombinant α-PrP adopts a PrP C -like conformation, while β-PrP conformationally resembles PrP Sc , to these we raised 81 monoclonal antibodies in Prnp 0/0 mice. The N-terminal residues 91-110 are highly immunogenic in β-PrP-immunised mice and of (17/41) anti-β-PrP antibodies that could be epitope-mapped, ∼ 70%, recognised this segment. In contrast, only 3/40 anti-α-PrP antibodies could be mapped and none interacted with this region, instead recognising residues 131-150, 141-160 and 171-190. Native PrP C was recognised by both antibody groups, but only anti-β-PrP antibodies directed to 91-110 residues recognised native PrP Sc with high affinity, where in addition, species heterogeneity was also evident. Within the six anti-β-PrP antibodies studied, they all recognised PK-treated native human and mouse PrP Sc , four failed to recognise PK-treated native bovine PrP Sc , one of which also did not recognise native PK-tre

Who reads β-PrP form of human prion protein stimulates production of monoclonal antibodies to epitope 91–110 that recognise native PrPSc?

Author

Azadeh Khalili-Shirazi; Maria Kaisar; Gary Mallinson; Samantha Jones; Daljit Bhelt; Carol Fraser; Anthony R. Clarke; Simon H. Hawke; Graham S. Jackson; John Collinge

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1570-9639)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)