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Decrease of energy spilling in Escherichia coli continuous cultures with rising specific growth rate and carbon wasting by Kaspar Valgepea; Kaarel Adamberg; Raivo Vilu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Background Growth substrates, aerobic/anaerobic conditions, specific growth rate (μ) etc. strongly influence __Escherichia coli__ cell physiology in terms of cell size, biomass composition, gene and protein expression. To understand the regulation behind these different phenotype properties, it is useful to know carbon flux patterns in the metabolic network which are generally calculated by metabolic flux analysis (MFA). However, rarely is biomass composition determined and carbon balance carefully measured in the same experiments which could possibly lead to distorted MFA results and questionable conclusions. Therefore, we carried out both detailed carbon balance and biomass composition analysis in the same experiments for more accurate quantitative analysis of metabolism and MFA. ## Results We applied advanced continuous cultivation methods (A-stat and D-stat) to continuously monitor __E. coli__ K-12 MG1655 flux and energy metabolism dynamic responses to change of μ and glucose-acetate co-utilisation. Surprisingly, a 36% reduction of ATP spilling was detected with increasing μ and carbon wasting to non-CO~2~ by-products under constant biomass yield. The apparent discrepancy be

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Author

Kaspar Valgepea; Kaarel Adamberg; Raivo Vilu

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2006]-; Springer Science and Business Media LLC (ISSN 1752-0509)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)