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Cellular Microarrays for Assessing Single-cell Phenotypic Changes in Vascular Cell Populations by E. Smith; M. Zagnoni; M. E. Sandison is a Engineering article available to read on EtoBox.

What is Cellular Microarrays for Assessing Single-cell Phenotypic Changes in Vascular Cell Populations about?

Microengineering technologies provide bespoke tools for single-cell studies, including microarray approaches. There are many challenges when culturing adherent single cells in confined geometries for extended periods, including the ability of migratory cells to overcome confining cell-repellent surfaces with time. Following studies suggesting clonal expansion of only a few vascular smooth muscle cells (vSMCs) contributes to plaque formation, the investigation of vSMCs at the single-cell level is central to furthering our understanding of atherosclerosis. Herein, we present a medium throughput cellular microarray, for the tracking of single, freshly-isolated vSMCs as they undergo phenotypic modulation __in vitro__. Our solution facilitates long-term cell confinement (> 3 weeks) utilising novel application of surface functionalisation methods to define individual culture microwells. We demonstrate successful tracking of hundreds of native vSMCs isolated from rat aortic and carotid artery tissue, monitoring their proliferative capacity and uptake of oxidised low-density lipoprotein (oxLDL) by live-cell microscopy. After 7 days __in vitro__, the majority of viable SMCs remained as sing

Who reads Cellular Microarrays for Assessing Single-cell Phenotypic Changes in Vascular Cell Populations?

Author

E. Smith; M. Zagnoni; M. E. Sandison

Publisher

Springer Science and Business Media LLC

Published

2023

Language

EN

Field

Engineering (Physical Sciences)