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In vitro inhibition of CYP3A4 by herbal remedies frequently used by cancer patients by Silje Engdal; Odd Georg Nilsen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The herbal remedies Natto K2, __Agaricus__, mistletoe, noni juice, green tea and garlic, frequently used by cancer patients, were investigated for their __in vitro__ inhibition potential of cytochrome P‐450 3A4 (CYP3A4) metabolism. To our knowledge, only garlic and green tea had available data on the possible inhibition of CYP3A4 metabolism. Metabolic studies were performed with human c‐DNA baculovirus expressed CYP3A4. Testosterone was used as a substrate and ketoconazole as a positive quantitative inhibition control. The formation of 6‐__β__‐OH‐testosterone was quantified by a validated HPLC methodology. Green tea was the most potent inhibitor of CYP3A4 metabolism (IC~50~: 73 μg/mL), followed by __Agaricus,__ mistletoe and noni juice (1324, 3594, >10 000 μg/mL, respectively). All IC~50~ values were high compared with those determined for crude extracts of other herbal remedies. The IC~50~/IC~25~ ratios for the inhibiting herbal remedies ranged from 2.15 to 2.67, indicating similar inhibition profiles of the herbal inhibitors of CYP3A4. Garlic and Natto K2 were classified as non‐inhibitors. Although __Agaricus__, noni juice, mistletoe and green tea inhibited CYP3A4 met

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Author

Silje Engdal; Odd Georg Nilsen

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-418X)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)