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Mitochondrial diversity evaluated by the single strand conformation polymorphism method in African and North American house flies ( Musca domestica L.) by J. G. Marquez; E. S. Krafsur is a Agricultural and Biological Sciences article available to read on EtoBox.

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## Abstract Single strand conformation polymorphisms (SSCPs) provide a convenient and inexpensive method of surveying mitochondrial genetic variation in large samples. We investigated how much variation should be incorporated into such surveys by scoring SSCP variation at eight mitochondrial loci in each of four sub‐Saharan African and four North American house fly (Diptera: Muscidae.) populations. Hierarchical analysis of diversity was performed on haplotype frequencies at each locus and on haplotype frequencies formed by combining haplotypes from two, three, four, five and eight loci. Composite haplotypes at two loci were as informative about population structure as those composed of a greater number of loci. Increasing the number of loci increased diversity estimates within, but not between, populations. Mean composite haplotype diversities (__16S2__ and __COII__) were 0.49 ± 0.09 among the African populations and 0.32 ± 0.08 among the North American populations. Only two of 16 haplotypes were shared between continents. Nei's genetic differentiation statistic between populations in continents __G__~PC~ was 0.30 ± 0.06 and mean genetic differentiation between continents __G__~CT~

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Author

J. G. Marquez; E. S. Krafsur

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0962-1075)

Published

2003

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)