Can I read Process Optimization of the Integrated Synthesis and Secretion of Ectoine and Hydroxyectoine Under Hyper/hypo-osmotic Stress on EtoBox?
Process Optimization of the Integrated Synthesis and Secretion of Ectoine and Hydroxyectoine Under Hyper/hypo-osmotic Stress by C. Fallet; P. Rohe; E. Franco-Lara is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Process Optimization of the Integrated Synthesis and Secretion of Ectoine and Hydroxyectoine Under Hyper/hypo-osmotic Stress about?
## Abstract The synthesis and secretion of the industrial relevant compatible solutes ectoine and hydroxyectoine using the halophile bacterium __Chromohalobacter salexigens__ were studied and optimized. For this purpose, a cascade of two continuously operated bioreactors was used. In the first bioreactor, cells were grown under constant hyperosmotic conditions and thermal stress driving the cells to accumulate large amounts of ectoines. To enhance the overall productivity, high cell densities up to 61 g L^−1^ were achieved using a cross‐flow ultrafiltration connected to the first bioreactor. In the coupled second bioreactor the concentrated cell broth was subjected to an osmotic and thermal down‐shock by addition of fresh distilled water. Under these conditions, the cells are forced to secrete the accumulated intracellular ectoines into the medium to avoid bursting. The cultivation conditions in the first bioreactor were optimized with respect to growth temperature and medium salinity to reach the highest synthesis (productivity); the second bioreactor was optimized using a multi‐objective approach to attain maximal ectoine secretion with simultaneous minimization of cell death and
Who reads Process Optimization of the Integrated Synthesis and Secretion of Ectoine and Hydroxyectoine Under Hyper/hypo-osmotic Stress?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- C. Fallet; P. Rohe; E. Franco-Lara
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)