Can I read A New Myocyte-specific Enhancer-binding Factor That Recognizes a Conserved Element Associated with Multiple Muscle-specific Genes. on EtoBox?

A New Myocyte-specific Enhancer-binding Factor That Recognizes a Conserved Element Associated with Multiple Muscle-specific Genes. by L A Gossett; D J Kelvin; E A Sternberg; E N Olson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is A New Myocyte-specific Enhancer-binding Factor That Recognizes a Conserved Element Associated with Multiple Muscle-specific Genes. about?

Exposure of skeletal myoblasts to growth factor-deficient medium results in transcriptional activation of muscle-specific genes, including the muscle creatine kinase gene (mck). Tissue specificity, developmental regulation, and high-level expression of mck are conferred primarily by a muscle-specific enhancer located between base pairs (bp) -1350 and -1048 relative to the transcription initiation site (E. A. Sternberg, G. Spizz, W. M. Perry, D. Vizard, T. Weil, and E. N. Olson, Mol. Cell. Biol. 8:2896-2909, 1988). To begin to define the regulatory mechanisms that mediate the selective activation of the mck enhancer in differentiating muscle cells, we have further delimited the boundaries of this enhancer and analyzed its interactions with nuclear factors from a variety of myogenic and nonmyogenic cell types. Deletion mutagenesis showed that the region between 1,204 and 1,095 bp upstream of mck functions as a weak muscle-specific enhancer that is dependent on an adjacent enhancer element for strong activity. This adjacent activating element does not exhibit enhancer activity in single copy but acts as a strong enhancer when multimerized. Gel retardation assays combined with DNase I

Who reads A New Myocyte-specific Enhancer-binding Factor That Recognizes a Conserved Element Associated with Multiple Muscle-specific Genes.?

Author

L A Gossett; D J Kelvin; E A Sternberg; E N Olson

Published

1989

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)