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The document discusses a method for the rapid purification of bispecific antibodies (BsAbs) through selective modulation of protein A binding by introducing specific mutations in the Fc region of human IgG1. The identified mutations (T307P, L309Q, and Q311R) enhance the interaction with the neonatal Fc receptor (FcRn) while disrupting protein A binding, allowing for efficient separation of BsAbs from parental antibodies. This approach facilitates high-throughput generation of BsAbs with normal serum half-li
- Author
- Dipak
- Language
- EN