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Can I read Determination of theophylline and paraxanthine in urine samples by liquid chromatography using the H-point standard additions method on EtoBox?
Determination of theophylline and paraxanthine in urine samples by liquid chromatography using the H-point standard additions method by Pilar Campíns-Falcó; Francisco Bosch-Reig; Rosa Herráez-Hernández; Adela Sevillano-Cabeza is a Chemistry article available to read on EtoBox.
What is Determination of theophylline and paraxanthine in urine samples by liquid chromatography using the H-point standard additions method about?
The simultaneous determmatlon of theophylhne and paraxanthme m urine samples by the H-pomt standard additions method (HPSAM) IS described Samples are extracted with Cl, solid-phase extraction cartndges and chromatographed using a Hypersd Cl,-ODS column and a mobile phase conslstmg of acetomtnle-phosphate buffer m the gradient elutlon mode Under these conditions theophylhne and paraxanthme are eluted with short retention times Although then chromatographlc peaks are overlapped and their spectra are very similar, the H-point standard addltlons method provides excellent results m the determmatlon of both xanthmes at therapeutic levels Keywords Liquid chromatography, H-point standard addltlon method, Paraxanthme, Theophylhne, Xanthmes Numerous hqmd chromatographlc (LC) methods for the determmatlon of theophylhne m blological fluids have been proposed Although theophylhne 1s easily detected when it IS present at therapeutic concentrations (l-20 pg ml-'), it has been reported that many exogenous and endogenous compounds Interfere [l] The mablhty to separate theophyllme from some of its metabohtes m a reasonable tune has been a typlcal problem m most of the LC procedures [2-71 The presenc
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It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Pilar Campíns-Falcó; Francisco Bosch-Reig; Rosa Herráez-Hernández; Adela Sevillano-Cabeza
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)
- Published
- 1992
- Language
- EN
- Field
- Chemistry (Physical Sciences)