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Inhibition of radiation induced migration of human head and neck squamous cell carcinoma cells by blocking of EGF receptor pathways by Anja C Pickhard; Johanna Margraf; Andreas Knopf; Thomas Stark; Guido Piontek; Carolin Beck; Anne-Laure Boulesteix; Elias Q Scherer; Steffi Pigorsch; Jürgen Schlegel; Wolfgang Arnold; Rudolf Reiter is a Medicine article available to read on EtoBox.
What is Inhibition of radiation induced migration of human head and neck squamous cell carcinoma cells by blocking of EGF receptor pathways about?
## Background Recently it has been shown that radiation induces migration of glioma cells and facilitates a further spread of tumor cells locally and systemically. The aim of this study was to evaluate whether radiotherapy induces migration in head and neck squamous cell carcinoma (HNSCC). A further aim was to investigate the effects of blocking the epidermal growth factor receptor (EGFR) and its downstream pathways (Raf/MEK/ERK, PI3K/Akt) on tumor cell migration in vitro. ## Methods Migration of tumor cells was assessed via a wound healing assay and proliferation by a MTT colorimeritric assay using 3 HNSCC cell lines (BHY, CAL-27, HN). The cells were treated with increasing doses of irradiation (2 Gy, 5 Gy, 8 Gy) in the presence or absence of EGF, EGFR-antagonist (AG1478) or inhibitors of the downstream pathways PI3K (LY294002), mTOR (rapamycin) and MEK1 (PD98059). Biochemical activation of EGFR and the downstream markers Akt and ERK were examined by Western blot analysis. ## Results In absence of stimulation or inhibition, increasing doses of irradiation induced a dose-dependent enhancement of migrating cells (p < 0.05 for the 3 HNSCC cell lines) and a decrease of cell proliferat
Who reads Inhibition of radiation induced migration of human head and neck squamous cell carcinoma cells by blocking of EGF receptor pathways?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Anja C Pickhard; Johanna Margraf; Andreas Knopf; Thomas Stark; Guido Piontek; Carolin Beck; Anne-Laure Boulesteix; Elias Q Scherer; Steffi Pigorsch; Jürgen Schlegel; Wolfgang Arnold; Rudolf Reiter
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2001]-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-2407)
- Published
- 2011
- Language
- EN
- ISBN
- 9781471240713
- Field
- Medicine (Health Sciences)