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Analysis of v‐Ha‐ ras and v‐ fos oncogene transduction into a mouse epidermal cell line with “initiated” phenotype in culture but normal skin phenotype in vivo by Masato Ueda; Hideki Kawamura; Christian Sutter; Adam Glick; Stuart H. Yuspa; James E. Strickland is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Analysis of v‐Ha‐ ras and v‐ fos oncogene transduction into a mouse epidermal cell line with “initiated” phenotype in culture but normal skin phenotype in vivo about?
## Abstract Cell line SCR722 was derived from adult SENCAR mouse epidermal cells initiated in culture by treatment with the carcinogen __N__‐methyl‐__N__′‐nitro‐N‐nitrosoguanidine and selection for foci proliferating in medium with calcium levels that induce terminal differentiation in normal cells. Expansion of one of these foci and two additional cell clonings produced cell line SCR722, which was near‐tetraploid and formed normal skin when grafted to athymic nude mouse hosts. However, unlike normal keratinocytes, SCR722 cells fail to suppress papilloma formation when grafted along with papilloma cell line SP‐1. For optimum growth in culture, SCR722 cells required fibroblast‐conditioned medium and 0.5 mM Ca^2+^. SCR722 cells had a wild‐type c‐Ha‐__ras__ gene but had lost their requirement for conditioned medium in culture and produced dysplastic papillomas in grafts when transduced with the v‐Ha‐__ras__ gene. SCR722 cells stably expressing the v‐__fos__ gene produced normal epidermis in grafts, but when these cells were transduced with the v‐Ha‐__ras__ gene, they produced carcinomas. Clones with greater expression of the transfected v‐__fos__ gene had a more invasive phenotype in
Who reads Analysis of v‐Ha‐ ras and v‐ fos oncogene transduction into a mouse epidermal cell line with “initiated” phenotype in culture but normal skin phenotype in vivo?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Masato Ueda; Hideki Kawamura; Christian Sutter; Adam Glick; Stuart H. Yuspa; James E. Strickland
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0899-1987)
- Published
- 1995
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)