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Can I read ^68^Ga-Labeled Maleimide for Blood Pool and Lymph PET Imaging through Covalent Bonding to Serum Albumin In Vivo on EtoBox?
^68^Ga-Labeled Maleimide for Blood Pool and Lymph PET Imaging through Covalent Bonding to Serum Albumin In Vivo by Lixia Feng; Jianyang Fang; Xinying Zeng; Huanhuan Liu; Jingru Zhang; Lumei Huang; Zhide Guo; Rongqiang Zhuang; Xianzhong Zhang is a Engineering article available to read on EtoBox.
What is ^68^Ga-Labeled Maleimide for Blood Pool and Lymph PET Imaging through Covalent Bonding to Serum Albumin In Vivo about?
This study aims to develop a novel ^68^Ga-labeled tracer, which can covalently bind to albumin in vivo based on the maleimide–thiol strategy, and to evaluate its potential applications using positron emission tomography (PET). ^68^Ga-labeled maleimide-monoamide-DOTA (denoted as [^68^Ga]Ga-DM) was prepared conveniently with a high radiochemical yield (>90%) and radiochemical purity (>99%). Its molar activity was calculated as 249.60 ± 68.50 GBq/μmol, and the octanol–water partition coefficient (Log__P__) was −3.15 ± 0.08 with good stabilities. In vitro experiments showed that [^68^Ga]Ga-DM can bind to albumin efficiently and rapidly, with a binding fraction of over 70%. High uptake and excellent retention in blood were observed with a long half-life (__t__~1/2Z~) of 190.15 ± 24.14 min, which makes it possible for blood pool PET imaging with high contrast. The transient micro-bleeding in the rat model was detected successfully with PET imaging. In addition, the uptakes of [^68^Ga]Ga-DM in the inflammatory popliteal lymph nodes depend on the severity (5.90% ID/g and 2.32% ID/g vs 1.01% ID/g for healthy lymph nodes at 0.5 h post-injection) indicating its feasibility for lymphatic imagi
Who reads ^68^Ga-Labeled Maleimide for Blood Pool and Lymph PET Imaging through Covalent Bonding to Serum Albumin In Vivo?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Lixia Feng; Jianyang Fang; Xinying Zeng; Huanhuan Liu; Jingru Zhang; Lumei Huang; Zhide Guo; Rongqiang Zhuang; Xianzhong Zhang
- Published
- 2022
- Language
- EN
- Field
- Engineering (Physical Sciences)