Opening book details…
Can I read Blockade by ferrous iron of Ca2+ influx through N-methyl-d-aspartate receptor channels in immature cultured rat cortical neurons on EtoBox?
Blockade by ferrous iron of Ca2+ influx through N-methyl-d-aspartate receptor channels in immature cultured rat cortical neurons by Noritaka Nakamichi; Hiroshi Ohno; Yoichi Nakamura; Takao Hirai; Nobuyuki Kuramoto; Yukio Yoneda is a Neuroscience article available to read on EtoBox.
What is Blockade by ferrous iron of Ca2+ influx through N-methyl-d-aspartate receptor channels in immature cultured rat cortical neurons about?
## Abstract Rat cortical neurons cultured for 3 days __in vitro__ were loaded with the fluorescent indicator fluo‐3 for assessment of intracellular free calcium ion (Ca^2+^) concentrations with the aid of a confocal laser‐scanning microscope. In the absence of added MgCl~2~, the addition of NMDA induced a rapid but sustained increase in the number of fluorescent neurons in a concentration‐dependent manner at a concentration range of 1–100 μm with the increase by KCl being transient. The addition of FeCl~2~, but not FeCl~3~, markedly inhibited the increase by NMDA in a reversible manner at concentrations of 10–200 μm, without affecting that by KCl. Extensive analyses revealed clear differentiation between inhibitions by ferrous iron and other channel blockers known to date. The inhibition by FeCl~2~ was completely prevented by the addition of two different iron chelators. Exposure to NMDA alone did not lead to cell death in immature cultured neurons, however, while further addition of FeCl~2~ invariably induced neuronal cell death 24 h after exposure. These results give support to our previous proposal that NMDA receptor complex may contain a novel site sensitive to blockade by ferr
Who reads Blockade by ferrous iron of Ca2+ influx through N-methyl-d-aspartate receptor channels in immature cultured rat cortical neurons?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Noritaka Nakamichi; Hiroshi Ohno; Yoichi Nakamura; Takao Hirai; Nobuyuki Kuramoto; Yukio Yoneda
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)
- Published
- 2002
- Language
- EN
- Field
- Neuroscience (Life Sciences)