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This document summarizes materials and methods used in a study involving optogenetic tools. It describes: 1) Reagents purchased including Bafilomycin A1, Tetrodotoxin, and Ascorbic acid. Cells were cultured in house. Constructs for CD63-PHluorin and Synaptophysin-pHluorin were obtained from other sources. 2) Plasmid constructs including Phoenix, which uses light to control acidification and neurotransmitter uptake. Arch-3 proton pumps were expressed in synaptic vesicles. pHluorin reported vesicle acidifi

Author

Chrisantus Okaka

Language

EN