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Changing fitness consequences of hsp70 copy number in transgenic Drosophila larvae undergoing natural thermal stress by S. P. Roberts; M. E. Feder is a Environmental Science article available to read on EtoBox.

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## Abstract 1. Transgenic manipulation of the gene copy number of __hsp70__, which encodes the major inducible heat‐shock protein of __Drosophila melanogaster__ (Hsp70), affects both Hsp70 levels and inducible thermotolerance in the laboratory; here parallel effects in transgenic __Drosophila__ larvae undergoing natural or simulated natural thermal stress are demonstrated. 2. Necrotic fruit was infested with larvae of either of two transgenic strains, one transformed with 12 extra copies of the __hsp70__ gene (extra‐copy strain) and a sister strain possessing only the wild‐type number (10) of __hsp70__ genes (excision strain), and then allowed to heat to variable extents. 3. As the intensity of thermal stress increased, the consequences of extra __hsp70__ copies reversed. After no or moderate thermal stress, excision larvae survived better than did extra copy larvae. By contrast, extra copy larvae tolerated intense hyperthermia better than did excision larvae. 4. These results establish that the Hsp70‐mediated enhancement of stress tolerance, previously demonstrated only for artificial stress regimes in the laboratory, extends to natural stress regimes. 5. Mortality due to overexpr

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Author

S. P. Roberts; M. E. Feder

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley; JSTOR (ISSN 0269-8463)

Published

2000

Language

EN

Field

Environmental Science (Physical Sciences)