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Pentose-phosphate pathway in Saccharomyces cerevisiae: analysis of deletion mutants for transketolase, transaldolase, and glucose 6-phosphate dehydrogenase by Ine Schaaff-Gerstenschlger; Friedrich K. Zimmermann is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Pentose-phosphate pathway in Saccharomyces cerevisiae: analysis of deletion mutants for transketolase, transaldolase, and glucose 6-phosphate dehydrogenase about?

Deletion mutants for the yeast transketolase gene TKL1 were constructed by gene replacement. Transketolase activity was below the level of detection in mutant crude extracts. Transketolase protein could be detected as a single protein band of the expected size by Western-blot analysis in wild-type strains but not in the deletion mutant. Deletion of TKL1 led to a reduced but distinct growth in synthetic medium without an aromatic amino-acid supplement. We also isolated double and triple mutants for transketolase (tkll), transaldolase (tail), and glucose 6-phosphate dehydrogenase (zwfl) by crossing the different mutants. A tall tkll double mutant grew nearly like wild-type in rich medium. Only the tkll zwfl double and the tall tkl! zwfl triple mutant grew more slowly than the wild-type in rich medium. This growth defect could be partly alleviated by the addition of xylulose but not ribose. The triple mutant still grew slowly on a synthetic mineral salts medium without a supplement of aromatic amino acids. This suggests the existence of an alternative but limited source of pentose phosphates and erythrose 4-phosphate in the tkl! zwf! double mutants. Hybridization with low stringency s

Who reads Pentose-phosphate pathway in Saccharomyces cerevisiae: analysis of deletion mutants for transketolase, transaldolase, and glucose 6-phosphate dehydrogenase?

Author

Ine Schaaff-Gerstenschlger; Friedrich K. Zimmermann

Publisher

Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0172-8083)

Published

1993

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)