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Development of a Common Biosensor Format for an Enzyme Based Biosensor Array to Monitor Fruit Quality by Shobha Jawaheer; S.F White; S.D.D.V Rughooputh; David C Cullen is a Engineering article available to read on EtoBox.

What is Development of a Common Biosensor Format for an Enzyme Based Biosensor Array to Monitor Fruit Quality about?

Individual enzyme-based biosensors involving three-electrode systems were developed for the detection of analytes comprising markers of the stage of maturity and quality in selected fruits of economic importance to tropical countries. Importantly, a common fabrication format has been developed to simplify manufacture and allow future integration of the individual sensors into a single multi-sensor array. Specifically, sensors for b-D-glucose, total D-glucose, sucrose and ascorbic acid have been developed. Pectin, a natural polysaccharide present in plant cells, was used as a novel matrix to enhance enzyme entrapment and stabilisation in the sensors. Except for ascorbic acid, all the sensors function via the detection of enzymatically generated H 2 O 2 at rhodinised carbon electrodes. Since ascorbic acid is electrochemically active at the working potential chosen ('/350 mV vs. Ag/AgCl), it was measured directly. Enzyme sensors demonstrated expected response with respect to their substrates, typically 0 Á/0.8 mA/20 mm 2 electrode area response over analyte ranges of 0 Á/7 mM. Interferences related to electrochemically active compounds present in fruits under study were significantly

Who reads Development of a Common Biosensor Format for an Enzyme Based Biosensor Array to Monitor Fruit Quality?

Author

Shobha Jawaheer; S.F White; S.D.D.V Rughooputh; David C Cullen

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)

Published

2003

Language

EN

Field

Engineering (Physical Sciences)