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Hydrolysis of dilute acid pretreated mixed hardwood and purified microcrystalline cellulose by cell-free broth from Clostridium thermocellum by L. R. Lynd; H. E. Grethlein is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The cellulase activity in cell-free broths from the thermophilic, ethanol-producing anaerobic bacterium Clos- tridium thermocellum is examined on both dilute-acidpretreated mixed hardwood 19OY0 maple, 10% birch) and Avicel. Experiments were conducted in vitro in order to distinguish properties of the cellulase from properties of the organism and to evaluate the effectiveness of &. thermocellum cellulase in the hydrolysis of a naturally oc- curring, lignin-containing substrate. The results obtained establish that essentially quantitative hydrolysis of cellulose from pretreated mixed hardwood is possible using this enzyme system. Pretreatment with 1% H2S04 and a 9-s residence time at 220, 210, 200, and 180°C allowed yields after enzymatic hydrolysis (percentage of glucan solubilizedlglucan potentially solubilized) of 97.8, 86.1, 82.0, and 34.6%, respectively. Enzymatic hydrolysis of mixed hardwood with no pretreatment resulted in a yield of 10.1%. Hydrolysis yields of >95% were obtained from -0.6 g/L mixed hardwood pretreated at 220°C in 7 h at broth strengths of 60 and 80% (vlv) and in approximately 48 h with 33% broth. Hydrolysis of pretreated mixed hardwood is compared to hydrolys

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Author

L. R. Lynd; H. E. Grethlein

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

1987

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)