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Immunogenicity of recombinant fragments of Plasmodium falciparum acidic basic repeat antigen produced in Escherichia coli by Ashima Kushwaha; Prakash P.L. Rao; R.padma Suresh; V.S. Chauhan is a Medicine article available to read on EtoBox.

What is Immunogenicity of recombinant fragments of Plasmodium falciparum acidic basic repeat antigen produced in Escherichia coli about?

The acidic basic repeat antigen (ABRA) of __Plasmodium falciparum__ is a potential vaccine candidate against erythrocytic stages of malaria. We report, for the first time, the immunological characteristics of recombinant ABRA constructs. The recombinant proteins representing different fragments of ABRA were expressed in __Escherichia coli__, either as fusions with maltose binding protein or as 6X histidine tagged molecules, and purified by affinity chromatography. Immunogenicity studies with these constructs in rabbits and mice indicated that the __N__‐terminal region is the least immunogenic part of ABRA. T‐cell proliferation experiments in mice immunized with these constructs revealed that the T‐cell epitopes were localized in the middle portion of the protein. More importantly, the purified immunoglobulin G specific to middle and __C__‐terminal fragments prevented parasite growth at levels approaching 80–90%. We found that these proteins were also recognized by sera from __P. falciparum__‐infected patients from Rourkela, a malaria endemic zone of India. Our immunogenicity results suggest that potential of ABRA as a vaccine candidate antigen should be investigated further.

Who reads Immunogenicity of recombinant fragments of Plasmodium falciparum acidic basic repeat antigen produced in Escherichia coli?

Author

Ashima Kushwaha; Prakash P.L. Rao; R.padma Suresh; V.S. Chauhan

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley; FASEB (ISSN 0141-9838)

Published

2001

Language

EN

Field

Medicine (Health Sciences)