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Independent effects of structural optimization and resveratrol functionalization on extracellular matrix scaffolds for bone ... 1 1 Introduction 1 2 Materials and methods 2 2.1 Scaffold fabrication 2 2.1.1 SIS scaffolds with different pore structures 2 2.1.2 RSV-functionalized SIS scaffolds 2 2.2 Scaffold characterization 2 2.2.1 Morphological and structure observations 2 2.2.2 Porosity 2 2.2.3 Water absorption ability 2 2.2.4 In vitro degradation 2 2.2.5 Drug loading and in vitro drug release 3 2.3 In vitro cell experiments 3 2.3.1 Cell culture 3 2.3.2 Cell proliferation 3 2.3.3 Cell viability 3 2.3.4 Alkaline phosphatase (ALP) staining and activity quantitation 3 2.3.5 Alizarin red S (ARS) staining and quantitation 3 2.3.6 Reverse transcription-quantitative PCR (qPCR) 3 2.3.7 Animal experiments 3 2.4 Statistical analysis 5 3 Results and discussion 5 3.1 Characterization of SIS scaffolds 5 3.1.1 Morphological observations 5 3.1.2 Pore size 5 3.1.3 Porosity 5 3.1.4 In vitro degradation 6 3.2 Characterization of RSV/SIS scaffolds 6 3.2.1 Morphological and structure observations 6 3.2.2 Drug loading and in vitro drug release 6 3.3 In vitro biocompatibility 6 3.4 In vitro osteogenic d
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- Language
- EN
- Category
- nonfiction
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