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Complete Amino-Acid Sequence of Bovine Seminal Ribonuclease, a Dimeric Protein from Seminal Plasma by Hisanori SUZUKI; Augusto PARENTE; Benedetta FARINA; Luigi GRECO; Renato LA MONTAGNA; Enzo LEONE is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Complete Amino-Acid Sequence of Bovine Seminal Ribonuclease, a Dimeric Protein from Seminal Plasma about?
The complete amino-acid sequence of BS-RNAse, a dimeric ribonuclease isolated from bovine seminal plasma, was determined. The reduced and S-carboxymethylated subunit chain of the enzyme was cleaved by trypsin and chymotrypsin. The resulting peptides, purified by cation-exchange chromatography were sequenced by dansyl-Edman, subtractive Edman degradation and carboxypeptidase A and B digestion. Chymotryptic peptides were used for the alignment. Automated Edman degradation of the native protein, through the N-terminal 41 amino-acid residues, completed the sequence information. The subunit chain of BS-RNAase, composed of 124 amino-acid residues, with a molecular mass of 13 610 Da, is highly homologous (81%) to pancreatic ribonuclease A. A good degree of homology (31 %) was also found with human angiogenin. No N-linked carbohydrate-attachment sites, such as Asn-X--Ser/Thr, were found in the protein. ## Die vollständige Aminosäuresequenz der dimeren Ribonuclease aus dem Seminalplasma des Rindes Zusammenfassung: Die vollständige
Who reads Complete Amino-Acid Sequence of Bovine Seminal Ribonuclease, a Dimeric Protein from Seminal Plasma?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Hisanori SUZUKI; Augusto PARENTE; Benedetta FARINA; Luigi GRECO; Renato LA MONTAGNA; Enzo LEONE
- Published
- 1987
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)