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Voltage-dependent K+ channels in the sarcolemma of mouse skeletal muscle by H. Brinkmeier; E. Zachar; R. Rüdel is a Medicine article available to read on EtoBox.
What is Voltage-dependent K+ channels in the sarcolemma of mouse skeletal muscle about?
The voltage-dependent K + channels of the mammalian sarcolemma were studied with the patchclamp technique in intact, enzymatically dissociated fibres from the toe muscle of the mouse. With a physiological solution (containing 2.5 mM K § in the pipette, depolarizing pulses imposed on a cell-attached membrane patch activated K + channels with a conductance of about 17 pS. No channel activity was observed when the pipette solution contained 2mM tetraethylammonium (TEA), or 2 mM 4-aminopyridine (4-AP). Whole cell recordings from these very small muscle fibres showed the well-known delayed rectifier K + outward current with a threshold of about -40 mY. The whole-cell current was completely blocked by 2 mM TEA in the bath, suggesting that the TEA-sensitive channels in the patch were also delayed rectifier channels. The inactivation properties of the channels were studied in the cell-attached mode. Averaged single-channel traces showed at least two types of channels discernible by their inactivation time course at a test potential of 60 mV. The fast type inactivated with a time constant of about 150ms, the slow type with a time constant of about 400 ms. A little channel activity always re
Who reads Voltage-dependent K+ channels in the sarcolemma of mouse skeletal muscle?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- H. Brinkmeier; E. Zachar; R. Rüdel
- Publisher
- Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0031-6768)
- Published
- 1991
- Language
- EN
- Field
- Medicine (Life Sciences)