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Can I read Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle on EtoBox?
Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle by Mijke W. Vogels; Bas W. M. van Balkom; Albert J. R. Heck; Cornelis A. M. de Haan; Peter J. M. Rottier; Joseph J. Batenburg; Dora V. Kaloyanova; J. Bernd Helms is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle about?
## Abstract To identify host factors involved in __Salmonella__ replication, SILAC‐based quantitative proteomics was used to investigate the interactions of __Salmonella typhimurium__ with the secretory pathway in human epithelial cells. Protein profiles of Golgi‐enriched fractions isolated from __S. typhimurium__‐infected cells were compared with those of mock‐infected cells, revealing significant depletion or enrichment of 105 proteins. Proteins annotated to play a role in membrane traffic were overrepresented among the depleted proteins whereas proteins annotated to the cytoskeleton showed a diverse behavior with some proteins being enriched, others being depleted from the Golgi fraction upon __Salmonella__ infection. To study the functional relevance of identified proteins in the __Salmonella__ infection cycle, small interfering RNA (siRNA) experiments were performed. siRNA‐mediated depletion of a selection of affected proteins identified five host factors involved in __Salmonella__ infection. Depletion of peroxiredoxin‐6 (PRDX6), isoform β‐4c of integrin β‐4 (ITGB4), isoform 1 of protein lap2 (erbin interacting protein; ERBB2IP), stomatin (STOM) or TBC domain containing protei
Who reads Quantitative proteomic identification of host factors involved in the Salmonella typhimurium infection cycle?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Mijke W. Vogels; Bas W. M. van Balkom; Albert J. R. Heck; Cornelis A. M. de Haan; Peter J. M. Rottier; Joseph J. Batenburg; Dora V. Kaloyanova; J. Bernd Helms
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)