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Specific interactions in complexes formed by polythiophene derivatives bearing polar side groups and plasmid DNA by Bruno Teixeira-Dias; Luis J. del Valle; Francesc Estrany; Elaine Armelin; Ramón Oliver; Carlos Alemán is a Materials Science article available to read on EtoBox.

What is Specific interactions in complexes formed by polythiophene derivatives bearing polar side groups and plasmid DNA about?

The interaction between plasmid DNA and polythiophene derivatives bearing substituents with polar groups has been examined using electrophoresis assays, and both UV-Vis and CD spectroscopies. Results clearly indicate that such conducting polymers form stable adducts with DNA, even although the interactions strongly depend on the chemical constitution of the polymers. Furthermore, digestion assays with EcoRI and BamHI evidence that the polymers form specific interactions with the DNA, protecting the target nucleotide sequences of these restriction enzymes. On the other hand, UV-Vis and CD spectra show that the interactions induce a fast and very significant exposition of the nitrogen bases, which is consequence of the structural alterations induced in the circular DNA. These results have been compared with those previously reported for polypyrrole, poly(3,4ethylenedioxythiophene) and poly(3-methylthiophene). Finally, a model based on the intercalation of the conducting polymer between the two DNA strands has been proposed.

Who reads Specific interactions in complexes formed by polythiophene derivatives bearing polar side groups and plasmid DNA?

Author

Bruno Teixeira-Dias; Luis J. del Valle; Francesc Estrany; Elaine Armelin; Ramón Oliver; Carlos Alemán

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0014-3057)

Published

2008

Language

EN

Field

Materials Science (Physical Sciences)