Can I read Delivery of CRISPR-Cas systems using phage-based vectors on EtoBox?

Delivery of CRISPR-Cas systems using phage-based vectors by Clément Fage; Nicolas Lemire; Sylvain Moineau is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Delivery of CRISPR-Cas systems using phage-based vectors about?

Antimicrobial resistance has spread quickly on a worldwide scale, reducing therapeutic options for bacterial infections. CRISPR-Cas is an adaptive immune system found in many prokaryotes that can be designed to target bacterial genomes, leading to cell death. Repurposing the CRISPR-Cas system as a therapeutic strategy offers an attractive way to overcome antimicrobial resistance. However, this strategy requires efficient vectors for the CRISPR-Cas system to reach the bacterial genomes. Engineered phages offer an attractive option as cargo delivery vectors. In this review, we discuss the production of phage-based vectors and the relevance of using repurposed CRISPR-Cas systems as antimicrobials. We also discuss recent progress in phage engineering that can potentially overcome the limitations and increase the efficiency of CRISPR-Cas delivery.

Who reads Delivery of CRISPR-Cas systems using phage-based vectors?

Author

Clément Fage; Nicolas Lemire; Sylvain Moineau

Publisher

Elsevier BV

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)