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Subunit specificity and interaction domain between GABA~A~ receptor‐associated protein (GABARAP) and GABA~A~ receptors by Jesper Nymann-Andersen; Hongbing Wang; Li Chen; Josef T. Kittler; Stephen J. Moss; Richard W. Olsen is a Neuroscience article available to read on EtoBox.
What is Subunit specificity and interaction domain between GABA~A~ receptor‐associated protein (GABARAP) and GABA~A~ receptors about?
GABARAP (GABA(A) receptor-associated protein) interacts with both microtubules and GABA(A) receptors in vitro and in vivo and is capable of modulating receptor channel kinetics. In this study, we use the intracellular loop of 15 GABA(A) receptor subunits to show that the interaction between GABARAP and GABA(A) receptor is specific for the gamma subunits. Pharmacological characterization of proteins purified by GABARAP affinity column indicates that native GABA(A) receptors interact with GABARAP. Quantitative yeast two-hybrid assays were used to identify the interaction domain in the gamma2 subunit for GABARAP binding, and to identify the interaction domain in GABARAP for GABA(A) receptor binding. A peptide corresponding to the GABARAP interaction domain in the gamma2 subunit was used to inhibit the interaction between GABARAP and the gamma2 subunit. In addition, the ability of GABARAP to promote cluster formation of recombinant receptors expressed in QT-6 fibroblasts was inhibited by a membrane-permeable form of this peptide in a time-dependent manner. The establishment of a model for GABARAP-induced clustering of GABA(A) receptors in living cells and the identification of subunit
Who reads Subunit specificity and interaction domain between GABA~A~ receptor‐associated protein (GABARAP) and GABA~A~ receptors?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Jesper Nymann-Andersen; Hongbing Wang; Li Chen; Josef T. Kittler; Stephen J. Moss; Richard W. Olsen
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)
- Published
- 2002
- Language
- EN
- Field
- Neuroscience (Life Sciences)