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Quality Assessment of Transcriptome Data Using Intrinsic Statistical Properties by Guillaume Brysbaert; François-Xavier Pellay; Sebastian Noth; Arndt Benecke is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Quality Assessment of Transcriptome Data Using Intrinsic Statistical Properties about?

In view of potential application to biomedical diagnosis, tight transcriptome data quality control is compulsory. Usually, quality control is achieved using labeling and hybridization controls added at different stages throughout the processing of the biologic RNA samples. These control measures, however, only reflect the performance of the individual technical manipulations during the entire process and have no bearing as to the continued integrity of the RNA sample itself. Here we demonstrate that intrinsic statistical properties of the resulting transcriptome data signal and signal-variance distributions and their invariance can be identified independently of the animal species studied and the labeling protocol used. From these invariant properties we have developed a data model, the parameters of which can be estimated from individual experiments and used to compute relative quality measures based on similarity with large reference datasets. These quality measures add supplementary, non-redundant information to standard quality control estimates based on spike-in and hybridization controls, and are exploitable in data analysis. A software application for analyzing datasets as w

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Author

Guillaume Brysbaert; François-Xavier Pellay; Sebastian Noth; Arndt Benecke

Publisher

Elsevier BV

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)