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Measurement of intracellular metabolites of primary metabolism and adenine nucleotides in chemostat cultivated \ \ Penicillium chrysogenum\ \ by U. Nasution; W.M. van Gulik; R.J. Kleijn; W.A. van Winden; A. Proell; J.J. Heijnen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Measurement of intracellular metabolites of primary metabolism and adenine nucleotides in chemostat cultivated \ \ Penicillium chrysogenum\ \ about?
## Abstract An experimental platform has been developed for rapid sampling and quenching of chemostat cultivated __Penicillium chrysogenum__ broth for metabolome analysis in highly dynamic experiments, aimed at the elucidation of the in vivo kinetic properties of metabolism. The sampling and quenching protocol available from __Saccharomyces cerevisiae__ had to be modified for __Penicillium chrysogenum__ mainly because of its filamentous character. Intracellular metabolites of glycolysis, TCA cycle, and adenine nucleotides were measured with isotope dilution mass spectrometry (IDMS) using a U‐^13^C‐labeled metabolite mix produced from yeast cells as internal standard. By addition of the U‐^13^C internal standard mix prior to the metabolite extraction procedure, partial degradation of metabolites as well as non‐linearity and drift of the LC‐MS/MS could be successfully compensated for. It was found that there is a serious matrix effect on metabolite extraction between different organisms, which is however completely corrected for by the IDMS approach. Intracellular metabolites could be analyzed with standard deviations of around 5%. A comparison of the metabolite levels between __Sacc
Who reads Measurement of intracellular metabolites of primary metabolism and adenine nucleotides in chemostat cultivated \ \ Penicillium chrysogenum\ \?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- U. Nasution; W.M. van Gulik; R.J. Kleijn; W.A. van Winden; A. Proell; J.J. Heijnen
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)
- Published
- 2006
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)