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Imaging of Lipids in Human Adipose Tissue by Cluster Ion TOF‐SIMS by Per Malmberg; Håkan Nygren; Katrin Richter; Yun Chen; Frida Dangardt; Peter Friberg; Ylva Magnusson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Imaging of Lipids in Human Adipose Tissue by Cluster Ion TOF‐SIMS about?

## Abstract Biopsies of human subcutaneous adipose tissue were taken from healthy donors. Samples were high‐pressure frozen, freeze‐fractured, and freeze dried. Imaging mass spectrometry of samples was performed in a TOF‐SIMS mass spectrometer equipped with a bismuth cluster ion source. Blood vessels, the connective tissue, and adipocytes can be seen in TOF‐SIMS images. Blood vessels were found labeled by a high content of sodium ions and potassium ions in their lumen and phosphocholine signal in smooth muscle cells of the vessel wall. The connective tissue showed high signal levels of CN^−^ fragments, derived from proteins and nucleic acids. Adipocytes showed high signal levels of phosphocholine and cholesterol ubiquitously in their membranes and diacylglycerols in some membrane sites. The central part of adipocytes showed high levels of triacylglycerols and fatty acids. These results are in accordance to those of biochemical studies; however, a precise spatial localization of lipids in adipocytes is demonstrated with MS imaging. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.

Who reads Imaging of Lipids in Human Adipose Tissue by Cluster Ion TOF‐SIMS?

Author

Per Malmberg; Håkan Nygren; Katrin Richter; Yun Chen; Frida Dangardt; Peter Friberg; Ylva Magnusson

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1059-910X)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)