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Can I read Three-Dimensional 13C Shift/1H–15N Coupling/15N Shift Solid-State NMR Correlation Spectroscopy on EtoBox?
Three-Dimensional 13C Shift/1H–15N Coupling/15N Shift Solid-State NMR Correlation Spectroscopy by Zhengtian Gu; Stanley J. Opella is a Chemistry article available to read on EtoBox.
What is Three-Dimensional 13C Shift/1H–15N Coupling/15N Shift Solid-State NMR Correlation Spectroscopy about?
Triple-resonance experiments capable of correlating directly bonded and proximate carbon and nitrogen backbone sites of uniformly 13 C-and 15 N-labeled peptides in stationary oriented samples are described. The pulse sequences integrate cross-polarization from 1 H to 13 C and from 13 C to 15 N with flip-flop (phase and frequency switched) Lee-Goldburg irradiation for both 13 C homonuclear decoupling and 1 H-15 N spin exchange at the magic angle. Because heteronuclear decoupling is applied throughout, the three-dimensional pulse sequence yields 13 C shift/ 1 H-15 N coupling/ 15 N shift correlation spectra with single-line resonances in all three frequency dimensions. Not only do the three-dimensional spectra correlate 13 C and 15 N resonances, they are well resolved due to the three independent frequency dimensions, and they can provide up to four orientationally dependent frequencies as input for structure determination. These experiments have the potential to make sequential backbone resonance assignments in uniformly 13 C-and 15 N-labeled proteins.
Who reads Three-Dimensional 13C Shift/1H–15N Coupling/15N Shift Solid-State NMR Correlation Spectroscopy?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Zhengtian Gu; Stanley J. Opella
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 1090-7807)
- Published
- 1999
- Language
- EN
- Field
- Chemistry (Physical Sciences)