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SPECTRIN‐LIKE PROTEINS IN GREEN ALGAE ( DESMIDIACEAE ) by Andreas Holzinger; Norbert DE Ruijter; Anne Mie Emons; Ursula LüTz‐Meindl is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is SPECTRIN‐LIKE PROTEINS IN GREEN ALGAE ( DESMIDIACEAE ) about?

Immunochemical detection of actin as well as spectrin-like proteins have been carried out in the green algae Micrasterias denticulata, Closterium lunula, and Euastrum oblongum. In these algae, actin is detected on Western blots at 43 kDa with antibodies to actin from higher plant and animal origin. By use of antibodies to human and chicken erythrocyte spectrin a cross-reactivity with desmid proteins is found at about the molecular mass of 220 kDa, where also human erythrocyte spectrin is detected. Additional bands are present at 120 kDa and 70 kDa, which are probably breakdown products. An antibody against chicken -actinin, a small protein of the spectrin superfamily, recognizes bands at 90 kDa, where it is expected, and 70 kDa, probably the same breakdown product as mentioned for spectrin. Isoelectric focusing provides staining at pI 4.6 with antibodies against spectrin. Immunogold labelling of spectrin and -actinin antigens on high-pressure frozen, freeze-substituted Micrasterias denticulata cells with the same antibodies exhibits staining, especially at membranes of different populations of secretory vesicles, at dictyosomes, and the plasma membrane. However, no clear correlatio

Who reads SPECTRIN‐LIKE PROTEINS IN GREEN ALGAE ( DESMIDIACEAE )?

Author

Andreas Holzinger; Norbert DE Ruijter; Anne Mie Emons; Ursula LüTz‐Meindl

Publisher

Elsevier Science; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 1065-6995)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)