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Analysis of insulin glulisine at the molecular level by X-ray crystallography and biophysical techniques by Gillis, Richard B. (author);Solomon, Hodaya V. (author);Govada, Lata (author);Oldham, Neil J. (author);Dinu, Vlad (author);Jiwani, Shahwar Imran (author);Gyasi-Antwi, Philemon (author);Coffey, Frank (author);Meal, Andy (author);Morgan, Paul S. (author);Harding, Stephen E. (author);Helliwell, John R. (author);Chayen, Naomi E. (author);Adams, Gary G. (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Analysis of insulin glulisine at the molecular level by X-ray crystallography and biophysical techniques about?
## Abstract This study concerns glulisine, a rapid-acting insulin analogue that plays a fundamental role in diabetes management. We have applied a combination of methods namely X-ray crystallography, and biophysical characterisation to provide a detailed insight into the structure and function of glulisine. X-ray data provided structural information to a resolution of 1.26 Å. Crystals belonged to the H3 space group with hexagonal (centred trigonal) cell dimensions a = b = 82.44 and c = 33.65 Å with two molecules in the asymmetric unit. A unique position of D21Glu, not present in other fast-acting analogues, pointing inwards rather than to the outside surface was observed. This reduces interactions with neighbouring molecules thereby increasing preference of the dimer form. Sedimentation velocity/equilibrium studies revealed a trinary system of dimers and hexamers/dihexamers in dynamic equilibrium. This new information may lead to better understanding of the pharmacokinetic and pharmacodynamic behaviour of glulisine which might aid in improving formulation regarding its fast-acting role and reducing side effects of this drug.
Who reads Analysis of insulin glulisine at the molecular level by X-ray crystallography and biophysical techniques?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Gillis, Richard B. (author);Solomon, Hodaya V. (author);Govada, Lata (author);Oldham, Neil J. (author);Dinu, Vlad (author);Jiwani, Shahwar Imran (author);Gyasi-Antwi, Philemon (author);Coffey, Frank (author);Meal, Andy (author);Morgan, Paul S. (author);Harding, Stephen E. (author);Helliwell, John R. (author);Chayen, Naomi E. (author);Adams, Gary G. (author)
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)