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Sensitive Fluorimetric Method for the Determination of Putrescine, Spermidine and Spermine by High-performance Liquid Chromatography and Its Application to Human Blood by Yukikazu Saeki; Noriyoshi Uehara; Shigeru Shirakawa is a Chemistry article available to read on EtoBox.

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suMMARY~ A fsst and sensitive methodfor the determination of putrescine, spermidiie and spermine by high-perfornuuxe liquid chromatography is described. These compounds are converted to their fluorescent dansy1 derivatives and are separated by a reversed-phase chromatographic system (Mieropak CH-10) with water and acetonitrile as mobile phase. The sensitivity of the method is 30 pmoles. The application of the method to the determination of polyamines in blood is described. It wss found that most of the poIyamines circulating in blood are localized in the erythrocytes, their content in normal human blood being spennidiie 14.1 + 3.1, and spermine 8.4 c 3.8 nmoles/ml packed erythrocytes. Putrescine is not present in normal human ee. The polyaniine level in serum is less than 0.1 nmoie/ml. The polyamine content of the erythrocytes from patients with malignant neopl-significantly elevated.

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Author

Yukikazu Saeki; Noriyoshi Uehara; Shigeru Shirakawa

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)

Published

1978

Language

EN

Field

Chemistry (Physical Sciences)