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This document outlines the protocol for antibody-oligonucleotide conjugation using NHS-PEG8-DBCO, detailing preparation, activation, purification, and analysis steps. It includes specific instructions on antibody desalting, DBCO activation, conjugation reaction, SDS-PAGE analysis, and BCA concentration assay. Each section provides calculations and procedural details necessary for successful conjugation and measurement of the resulting product.

Author
habibmuhammadedu
Language
EN

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