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Isolation and characterization of three families of auxin down-regulated cDNA clones by Neeraj Datta; Peter R. LaFayette; Philip A. Kroner; Ronald T. Nagao; Joe L. Key is a Agricultural and Biological Sciences article available to read on EtoBox.

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Five cDNA clones (ADR6, ADR11-1, ADR11-2, ADR12-1 and ADR12-2), representing three families of auxin down-regulated (ADR) genes were isolated and characterized. These were isolated by screening a 2Zap cDNA library with the partial cDNA clones p6, pl 1 and p12, isolated earlier (Baulcombe and Key, J Biol Chem 255: [8907][8908][8909][8910][8911][8912][8913] 1980). Hybrid-select translation of ADR6, ADR11-2 and ADR12-2 clones produced polypeptides of 33 kDa 22.5 kDa and a 6 and 7 kDa respectively, when analyzed by SDS-PAGE. ADR6 and ADR12-2 gave one and two spots, respectively, on an IEF-SDS 2D gel. ADR11-2 probably encodes a basic protein as it was only resolved on non-equilibrium pH gradient gel electrophoresis (NEPHGE). Genomic Southern blot analysis of ADR6, ADR11 and ADR12 suggests that each represents a small multigene family. The RNA levels corresponding to ADR6, ADR11 and ADR12 decrease in response to applied auxin by 100-, 15-and 10-fold, respectively . Runoff transcription, done in the presence and absence of auxin, showed that the rate of transcription of the genes corresponding to ADR6, ADR11-2 and ADR12-2 was reduced in the presence of auxin, but the decrease was small re

Who reads Isolation and characterization of three families of auxin down-regulated cDNA clones?

Author

Neeraj Datta; Peter R. LaFayette; Philip A. Kroner; Ronald T. Nagao; Joe L. Key

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0167-4412)

Published

1993

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)